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This Headstage Amplifier Cable kit is designed for use with the Pine Research WaveNeuro Fast-Scan Cyclic Voltammetry Potentiostat System.  The kit includes the DB-25 headstage cable with CP-305 bipolar stimulation connector, overall 36" long, five replaceable 2.5" microelectrode-headstage couplers, and 200 nA/V, working driven headstage amplifier.  The modular design of the Pine Research headstage kits allows researchers to diagnose cable issues in a piecewise manner.  Say goodbye to that box of headstages with questionable behaviors!

The headstage kit includes the cable, microelectrode-to-headstage couplers, and headstage amplifier.  Cables are available in 24" and 36" and couplers in 1.5" and 2.5".  The most common configurations are presented below, but additional lengths are available separately.

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# Description Price in USD
For use with Pine Research WaveNeuro and UNC UEI Systems
[NEC-HS2]

Headstage Amplifier 36" Cable Kit
kit includes UEI-compatible potentiostat to headstage cable with stimulation connector, overall 36" long, five replaceable 2.5" microelectrode-headstage couplers, and working driven headstage amplifier

Headstage Kit includes the items listed below
RRHC0136-T

Headstage Amplifier Cable
36" long, WaveNeuro and UEI Potentiostat compatible, includes CP-305 stimulator connector

AC01HS1

FSCV Headstage Amplifier
5 MΩ; 200 nA/V, working electrode driven

NOTE: A working electrode driven systems means the waveform is applied to the working electrode, which is then later software subtracted. Such a design is consistent with the general FSCV research community.

AC01HC0325-5

Microelectrode-Headstage Coupler - 2.5"
white reference, yellow working, pack of 5

Pine Research currently offers working driven headstage amplifiers.  In a working driven system, the reference electrode is grounded.  The FSCV potential waveform (ramp) is connected to the non-inverting input of the operational amplifier, while the working electrode is connected to the inverting input.  In this arrangement, the voltage at the microelectrode will follow the ramp applied to the inverting input.1

Even when no electrodes are connected (connector just in air) to the headstage amplifier cable, which is connected to the WaveNeuro, HDCV will show a current response that follows the applied waveform.  As described below, this is expected.

In this two-electrode configuration, current arising from electron-transfer reactions, such as the oxidation of dopamine, passes between reference and working electrodes.  The measured current passes through the headstage amplifer, where it is converted to voltage, and sums with the the ramp voltage at the inverting input. Mathematically,

V_O=-(i_{in}\times R_F)+V_R

where V_O is the output voltage, i_{in} is input current, R_F is feedback resistor (gain), and V_R is the CV ramp voltage.  By rearrangement, the signal voltage (proportional to the current across the 5\: M\Omega feedback resistor in the 200\:nA/V headstage) is then

(V_R-V_O)=i_{in}\times R_F=V_{signal}

HDCV software, which supports the WaveNeuro FSCV Potentiostat system, performs software subtraction of the ramp according to this relationship, resulting in only the true differential current measurement.1

(1) Takmakov, P.; McKinney, C. J.; Carelli, R. M.; Wightman, R. M. Instrumentation for Fast-Scan Cyclic Voltammetry Combined with Electrophysiology for Behavioral Experiments in Freely Moving Animals. Rev. Sci. Instrum. 2011, 82, 74302.

R.M. Wightman et. al. have reported on this topic in depth   If, after reviewing this document, you have any questions about our neuroelectrochemical research products, please do not hesitate to contact us.

All specifications are subject to change without notice.
Document # Title

DRU10120

WaveNeuro Fast Scan Cylic Voltammetry Potentiostat System User Guide
click here to download (4 MB download)

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